Journal: bioRxiv

Article Title: MK2-deficient mice are bradycardic and display delayed hypertrophic remodelling in response to a chronic increase in afterload

doi: 10.1101/2020.01.23.916049

Figure Lengend Snippet: (A) In vivo heart rate (HR) assessment by surface electrocardiography (ECG) in MK2 +/+ (n = 7) and MK2 −/− (n = 7) mice anesthetized with isoflurane. Arrow indicates injection of isoproterenol (ISO, 0.1 mg/kg). * P < MK2 +/+ vs. MK2 −/− , repeated-measures two-way ANOVA with Bonferroni post-test. (B) Representative electrocardiograms obtained by radio telemetry in conscious unrestrained MK2 +/+ (upper) and MK2 −/− (lower) mice. (C) Mean heart rates obtained from ECGs recorded over a 48-h time period by radio telemetry in conscious unrestrained MK2 +/+ (n = 6) and MK2 −/− (n = 6) mice. Data was extracted and analyzed in 3 h intervals and then averaged separately for each mouse prior to calculating the mean heart rate for each group. (D) Heart rates obtained from ECGs recorded over a 48-h time period by radio telemetry in conscious unrestrained MK2 +/+ (n = 6) and MK2 −/− (n = 6) mice. Data was extracted and analyzed in 3 h intervals. Mice were maintained on a 12:12 hour light:dark cycle (6h00:18h00) with the bar at the top of the graph indicating light and dark cycles. The circadian rhythm is clearly visible. (E) R-R interval and (F) P-R segment duration obtained from ECGs recorded over a 48-h time period by radio telemetry in conscious unrestrained MK2 +/+ (n = 6) and MK2 −/− (n = 6) mice. Data was extracted and analyzed in 3 h intervals and then values for light and dark cycles averaged separately for each mouse prior to calculating the mean heart rate for each group. (G) Mean 48 h SD1/SD2 ratios for MK2 +/+ (n = 6) and MK2 −/− (n = 6) mice where SD1 represents the standard deviation on the short axis and SD2 represents the standard deviation on the long axis of an ellipse fit to a plot of R-R n versus R-R n+1 . Data are expressed as mean ± SEM. * P < 0.05, ** P < 0.01.

Article Snippet: One-week post-surgery, data acquisition was initiated at a sampling frequency of 1 kHz and maintained for 48 h. Recordings were analysed with ECG Auto (version 2.8; EMKA Technologies, Paris, France).

Techniques: In Vivo, Injection, Standard Deviation

Journal: Research Reports: Health Effects Institute

Article Title: Multicenter Ozone Study in oldEr Subjects (MOSES): Part 1. Effects of Exposure to Low Concentrations of Ozone on Respiratory and Cardiovascular Outcomes

doi:

Figure Lengend Snippet: Schedule of Procedures

Article Snippet: Dr. Zareba has directed analyses of ECG recordings for studies examining associations between markers of HRV, repolarization, and other parameters and increased air pollutant concentrations, working with researchers at both the University of Rochester and Helmholtz Zentrum München, Germany.

Techniques: Pregnancy Test (hCG) Assay, Activity Assay, Clinical Proteomics

Journal: Research Reports: Health Effects Institute

Article Title: Multicenter Ozone Study in oldEr Subjects (MOSES): Part 1. Effects of Exposure to Low Concentrations of Ozone on Respiratory and Cardiovascular Outcomes

doi:

Figure Lengend Snippet: Overview of Procedures Performed on the Days Before, During, and After Exposure a

Article Snippet: Dr. Zareba has directed analyses of ECG recordings for studies examining associations between markers of HRV, repolarization, and other parameters and increased air pollutant concentrations, working with researchers at both the University of Rochester and Helmholtz Zentrum München, Germany.

Techniques: Activity Assay

Journal: Research Reports: Health Effects Institute

Article Title: Multicenter Ozone Study in oldEr Subjects (MOSES): Part 1. Effects of Exposure to Low Concentrations of Ozone on Respiratory and Cardiovascular Outcomes

doi:

Figure Lengend Snippet: Overview of Ozone Effects in MOSES

Article Snippet: Dr. Zareba has directed analyses of ECG recordings for studies examining associations between markers of HRV, repolarization, and other parameters and increased air pollutant concentrations, working with researchers at both the University of Rochester and Helmholtz Zentrum München, Germany.

Techniques: